Title

The effect of nitrogen source and concentration, medium pH and buffering on in vitro shoot growth and rooting in Eucalyptus marginata

Document Type

Journal Article

Publisher

Elsevier Ltd.

Faculty

Computing, Health and Science

School

Natural Sciences, Centre for Ecosystem Management

RAS ID

5077

Comments

This article was originally published as: Woodward, A. J., Bennett, I. J., & Pusswonge, S. (2006). The effect of nitrogen source and concentration, medium pH and buffering on in vitro shoot growth and rooting in Eucalyptus marginata. Scientia horticulturae, 110(2), 208-213. Original available here

Abstract

This work examined the effect of nitrogen source and medium buffering on the micropropagation of Eucalyptus marginata Donn ex Sm. The number of shoots was increased when media contained 2-(N-morpholino) ethanesulfonic acid (MES) but this increase was minor and only applied to one of the two clones tested. Highest root production was obtained when the medium contained 7.5 mM nitrogen in a ratio of 2NO3:1NH4+ and was buffered with 10 mM MES. In the rooting medium the pH was influenced most significantly by the nitrogen source, and then whether the medium was buffered. The media pH remained relatively constant when nitrate was the sole nitrogen source and this was assisted by the addition of 10 mM MES. Lower concentrations (<10 >mM) of MES were less effective in buffering media over a four-week culture period in both shoot multiplication and rooting medium.

DOI

10.1016/j.scienta.2006.07.005

 

Link to publisher version (DOI)

10.1016/j.scienta.2006.07.005