Beta amyloid and hyperphosphorylated tau deposits in the pancreas in type 2 diabetes
Computing, Health and Science
Exercise, Biomedical & Health Science/Centre of Excellence for Alzheimer's Disease Research and Care
Strong epidemiologic evidence suggests an association between Alzheimer disease (AD) and type 2 diabetes, To determine if amyloid beta (AP) and hyperphosphorylated tau occurs in type 2 diabetes, pancreas tissues from 21 autopsy cases (IO type 2 diabetes and 11 controls) were analyzed. APP and tau mRNAs were identified in human pancreas and in cultured insulinoma beta cells (INS~ I) by RT-PCR. Prominent APP and tau bands were detected by Western blotting in pancreatic extracts. Aggregated AB. hyperphosphorylated rau, ubiquilin, apolipoprotein E. apolipoprotein(a), IBI/JlP-1 and JNKI were detected in Langerhans islets in type 2 diabetic patients. AB was co-localized with amylin in islet amyloid deposits. In situ beta sheet formation of islet amyloid deposits was shown by infrared microspectroscopy (SIRMS). LPS increased APP in non-neuronal cells as well. We conclude that AP deposits und hyperphosphorylated tau are also associated with type 2 diabetes, highlighting common pathogenetic features in neurodegenerative disorders, including AD and type 2 diabetes und suggesting that AB deposits and hyperphosphorylated tau may also occur in other organs than the brain.
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