Title

Replacing dietary carbohydrate with protein and fat decreases the concentrations of small LDL and the inflammatory response induced by atherogenic diets in the guinea pig

Document Type

Journal Article

Publisher

Elsevier

Faculty

Computing, Health and Science

School

Exercise, Biomedical and Health Science

RAS ID

5447

Comments

This article was originally published as: Sharman, M. J., Fernandez, M. L., Zern, T. L., Torres-Gonzalez, M., Kraemer, W. J., & Volek, J. S. (2008). Replacing dietary carbohydrate with protein and fat decreases the concentrations of small LDL and the inflammatory response induced by atherogenic diets in the guinea pig. The Journal of Nutritional Biochemistry, 19(11), 732-738. Original article available here

Abstract

Guinea pigs resemble humans in cholesterol and lipoprotein metabolism; however, there is limited information on the vascular inflammatory response with induction of atherosclerosis in this animal model. The purpose of this study was to document a vascular inflammatory response associated with dietary-induced atherosclerosis in the guinea pig and determine the effect of replacing dietary carbohydrate with protein and fat on this response. Thirty male Hartley guinea pigs were randomly assigned to a high dietary cholesterol, high-carbohydrate (HC); a high-cholesterol, low-carbohydrate (LC) or a control (CON) diet for 12 weeks. Analysis of cytokine protein expression [interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-6 and GM-CSF) and m RNA expression (IFN-γ, TNF-α, IL-1β, MCP-1 and IL-8] were performed along with the measurement of cholesterol concentration in the aorta, plasma lipids and plasma low-density lipoprotein subfractions. There was a similar and significant accumulation of cholesterol in the thoracic aorta in the HC and LC groups compared to the CON group. Aortic cytokine protein expression (TNF-α, IFN-γ and IL-6) and m RNA expression (TNF-α and IFN-γ) were significantly elevated in both high-cholesterol fed groups (HC and LC) (P

DOI

10.1016/j.jnutbio.2007.09.008

 

Link to publisher version (DOI)

10.1016/j.jnutbio.2007.09.008