Title

Oligomerization and Toxicity of Aβ Fusion Proteins

Document Type

Journal Article

Publisher

Academic Press

Faculty

Faculty of Computing, Health and Science

School

School of Medical Sciences / Centre of Excellence for Alzheimer's Disease Research and Care

RAS ID

12475

Comments

This article was originally published as: Caine, J., Bharadwaj, P. , Sankovich, S., Ciccotosto, G., Streltsov, V., & Varghese, J. (2011). Oligomerization and toxicity of Aβ fusion proteins. Biochemical and Biophysical Research Communications, 409(3), 477-482. Original article available here

Abstract

This study has found that the Maltose binding protein Aβ42 fusion protein (MBP-Aβ42 ) forms soluble oligomers while the shorter MBP-Aβ16 fusion and control MBP did not. MBP-Aβ42. but neither MBP-Aβ16 nor control MBP, was toxic in a dose-dependent manner in both yeast and primary cortical neuronal cells, This study demonstrates the potential utility of MBP-Aβ42 as a reagent for drug screening assays in yeast and neuronal cell cultures and as a candidate for further Aβ42 characterization.

 

Link to publisher version (DOI)

10.1016/j.bbrc.2011.05.029