Title

A preliminary search for alternatives to albumin as a medium supplement for the culture of human sperm

Document Type

Journal Article

Faculty

Faculty of Computing, Health and Science

School

School of Medical Sciences / Systems and Intervention Research Centre for Health

RAS ID

15008

Comments

This article was originally published as: Matson, P. L., & Tardif, S. (2012). A preliminary search for alternatives to albumin as a medium supplement for the culture of human sperm. Reproductive Biology, 12(3), 329-331. Original article available here

Abstract

Non-animal macromolecules (Select Phytone™ UF, wheat peptone, dextran 40, hydroxyethyl starch and methyl cellulose) as an alternative medium supplement for human spermatozoa were compared to bovine serum albumin. Select Phytone™ UF and wheat peptone discolored the medium and smelled like broth, making them unlikely to be acceptable for clinical use, whilst the others were colorless and odorless. All supplements were effective in the yield of spermatozoa isolated by swim-up technique, and maintenance of sperm motility. In summary, there are non-animal macromolecules that will support short-term sperm culture.

DOI

10.1016/j.repbio.2012.09.006

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