Document Type

Journal Article

Publisher

PLOS

Faculty

Faculty of Computing, Health and Science

School

School of Medical Sciences

RAS ID

14975

Comments

This article was originally published as: Horvat, T., Dezeljin, M., Redzic, I., Barisic, D., Bosnar, M.H.., & Lauc, G. (2013). Reversibility of membrane N-Glycome of HeLa upon treatment with epigenetic inhibitors. PLoS One, 8(1), e54672 . Original article available here

Abstract

Glycans are essential regulators of protein function and are now in the focus of research in many physiological and pathophysiological processes. There are numerous modes of regulating their biosynthesis, including epigenetic mechanisms implicated in the expression of glyco-genes. Since N-glycans located at the cell membrane define intercellular communication as well as a cellular response to a given environment, we developed a method to preferentially analyze this fraction of glycans. The method is based on incorporation of living cells into polyacrylamide gels, partial denaturation of membrane proteins with 3 M urea and subsequent release of N-glycans with PNGase F followed by HPLC analysis. Using this newly developed method, we revealed multiple effects of epigenetic inhibitors Trichostatin A, sodium butyrate and zebularine on the composition of N-glycans in human cells. The induced changes were found to be reversible after inhibitor removal. Given that many epigenetic inhibitors are currently explored as a therapeutic strategy in treatment of cancer, wherein surface glycans play an important role, the presented work contributes to our understanding of their efficiency in altering the N-glycan profile of cancer cells in culture.

DOI

10.1371/journal.pone.0054672

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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