Document Type

Journal Article

Publication Title

Food Chemistry

Volume

417

Publisher

Elsevier

School

School of Science

RAS ID

57901

Funders

Research Plus CERC postdoctoral fellowship by CSIRO, Australia

Comments

Nelis, J. L., Dawson, A. L., Bose, U., Anderson, A., Colgrave, M. L., & Broadbent, J. A. (2023). Safe food through better labelling: A robust method for the rapid determination of caprine and bovine milk allergens. Food Chemistry, 417, Article 135885.

https://doi.org/10.1016/j.foodchem.2023.135885

Abstract

Accidental milk cross-contamination is one of the most common causes for costly food recalls. Yet, quantifying trace-levels of allergen is time-consuming and current methods are not adapted for routine analyses making quality control for trace-level allergen content impractical. This perpetuates voluntary “may-contain” statements that are unhelpful for people suffering from food allergies. Here, we developed a rapid LC-MS method enabling milk allergen quantification by comparing all tryptic-peptides of major milk allergens. The bovine-specific αS-2 casein peptide and allergen-epitope NAVPITPTLNR provided excellent performance in sensitivity (LOD 1 mg.kg − 1; LOQ 2 mg.kg − 1) across various dairy products, good recovery rates in baked croissants (77 % with a 10 % inter-day RSD) and a linear range of 2 – 2,000 mg.kg − 1. The method can be used for routine determination of trace-contamination with bovine milk allergen and the adulteration of high-value caprine dairy products with lower-value bovine milk products, protecting consumer trust and the growing population suffering from food allergies.

DOI

10.1016/j.foodchem.2023.135885

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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