Title

Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts

Document Type

Journal Article

Publisher

Cambridge University Press

School

Centre for Marine Ecosystems Research / School of Natural Science

RAS ID

21127

Comments

Originally published as: Fromont, J., Huggett, M.J., Lengger, S.K., Grice, K., Schönberg, C.H.L. (2015). Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts in Journal of the Marine Biological Association of the United Kingdom, 96(Special issue 2), 541-552. Available here.

Abstract

The biology and ecology of calcarean sponges are not as well understood as they are for demosponges. Here, in order to gain new insights, particularly about symbiotic relationships, the calcarean sponge Leucetta prolifera was sampled from south-western Australia and examined for its assumed photosymbionts. Pulse amplitude modulated fluorometry and extraction of photopigments established that the sponge was photosynthetic. Molecular analysis of the bacterial symbionts via sequencing of the V1–V3 region of the 16S rDNA gene confirmed that between 5 and 22% of all sequences belonged to the phylum Cyanobacteria, depending on the individual sample, with the most dominant strain aligning with Hormoscilla spongeliae, a widely distributed sponge symbiont. Analysis of fatty acids suggested that the sponge obtains nutrition through photosynthates from its symbionts. The relationship is assumed to be mutualistic, with the sponge receiving dietary support and the cyanobacteria sheltering in the sponge tissues. We list all Calcarea presently known to harbour photosymbionts.

DOI

10.1017/S0025315415000491