Title

Characterising the effect of geometry on a microchamber for producing controlled concentration gradients

Document Type

Journal Article

Publisher

Elsevier

Faculty

Faculty of Health, Engineering and Science

School

School of Engineering

RAS ID

18734

Comments

This article was originally published as: Glover K., Strang J., Lubansky A. (2014). Characterising the effect of geometry on a microchamber for producing controlled concentration gradients. Chemical Engineering Science, 117(), 389-395. Original article available here

Abstract

A microfluidic diffusion chamber with 3 inlets and a circular central chamber allows a 2D concentration gradient to develop. This diffusion chamber has been characterised numerically for the effect geometry has on equilibrium time, the concentration profile and the flow profile within the central chamber. As the Einstein-Smoluchowski relation predicts, the time to reach steady state is proportional to the square of the radius of the chamber but features within the chamber are qualitatively insensitive to the size of the chamber within the range of 100-1000. μm. Inlet width had a much more significant effect on the qualitative behaviour within the chamber, affecting the symmetry of the concentration profile. It is recommended that inlet widths are less than 80. μm to preserve symmetry. In this paper, the effect of geometry on both transient and steady-state behaviour has been explored, providing a basis and criteria for designing chambers for a wide range of applications, including studying the effect of concentration gradients on cell mobility or a rapid assay for biofilm development in a range of concentrations.

DOI

10.1016/j.ces.2014.06.045

Access Rights

Not open access

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