Date of Award

1996

Degree Type

Thesis

Degree Name

Bachelor of Science Honours

Faculty

Faculty of Science, Technology and Engineering

First Advisor

Dr Mary Boyce

Second Advisor

Dr Ian Bennett

Abstract

Several buffers were examined for their ability to separate a complex mixture of phenolic compounds using micellar electrokinetic capillary chromatography (MEKC). The phenolic mixture included simple phenols, phenolic acids and coumarins. Of the different buffers examined, 6 mM borate, 10 mM phosphate, 100 mM SDS, pH 8.3 gave the best separation. The ability of organic modifiers, complexation agents such as α cyclodextrin, β cyclodextrin and mixed SDS I Brij 35 micelles to improve the separation and peak retention time reproducibility of the buffer system was investigated. Baseline separation of the 18 phenolics was achieved by the 6 mM borate, 10 mM phosphate, I 00 mM SDS, 15 mM Brij 35, 10% acetonitrile, pH 8.3 buffer. However this buffer system had poor peak retention time reproducibility. The MEKC system was used to analyse the phenolic content of a range of eucalypt species. Through spiking, several phenolic and related compounds were identified. These compounds included gallic acid, ferulic acid, p-coumaric acid and shikimic acid. GC-MS and HPLC supported the results obtained by CE.

Included in

Plant Biology Commons

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