Factors influencing serum caffeine concentrations following caffeine ingestion

Document Type

Journal Article




Faculty of Health, Engineering and Science


ECU Health and Wellness Institute/ECU Health and Wellness Institute




This article was originally published as: Skinner, T., Jenkins, D., Leveritt, M., McGorm, A., Bolam, K., Coombes, J., & Taaffe, D. (2014). Factors influencing serum caffeine concentrations following caffeine ingestion. Journal of Science and Medicine in Sport, 17(5), 516-520. Original article available here


Objectives: To determine whether differences in training status, body composition and/or habitual caffeine intake influenced serum caffeine concentrations following caffeine ingestion. Design: Single-blind. Methods: Trained cyclists/triathletes (n = 14) and active (n = 14) males consumed 6 mg kg-1 anhydrous caffeine. Peak, total and time to peak serum caffeine concentrations were determined from venous blood samples at baseline and 6 time-points over 4 h following intake. Body composition was assessed by dual energy X-ray absorptiometry and habitual caffeine intake by a questionnaire. Results: Trained cyclists/triathletes had 16% lower peak caffeine concentrations following caffeine ingestion compared to active individuals, although this was not statistically significant (p = 0.066). There was no significant difference between trained cyclists/triathletes and active males in total (p = 0.131) or time to peak (p = 0.249) serum caffeine concentrations. Fat mass was significantly associated with total (r = 0.427, p = 0.038) but not peak (r = 0.343, p = 0.101) or time to peak serum caffeine concentration (β = 0.00008, p = 0.961). There were no associations between habitual caffeine intake and peak, total or time to peak serum caffeine concentrations. Conclusions: Following caffeine ingestion three findings from the study were evident: (1) endurance-trained athletes trended towards lower peak caffeine concentrations compared to active males; (2) higher fat mass was associated with higher concentrations of caffeine in the blood over 4 h, and (3) habitual caffeine intake does not appear to influence serum caffeine concentrations. Identification of the optimal conditions to ensure peak availability of caffeine within the blood and/or overcoming some of the variation in how individuals respond to caffeine requires consideration of the training status and body composition of the athlete.