Intracytoplasmic sperm injection using hyaluronicacid or polyvinylpyrrolidone: a time-lapse siblingoocyte study
Taylor & Francis
Place of Publication
School of Medical and Health Sciences
This study evaluated the effect of sperm selection and intracytoplasmic sperm injection (ICSI) on subsequent fertilization and embryo development using the hyaluronic acid-based SpermSlowTM (HA-ICSI) compared to injection with polyvinylpyrrolidone (PVP-ICSI). A total of 206 metaphase II oocytes were collected from 21 prospectively enrolled ICSI cycles at Fertility North between July 2014 and March 2015. Sibling oocytes were randomized into HA-ICSI and PVP-ICSI (n¼103 per group). Subsequent fertilization outcomes and embryo development in terms of qualitative and quantitative time-lapse measures following three-day culture in the EmbryoscopeTM were compared. HA-ICSI resulted in significantly lower abnormal fertilization rates (1.9% vs 9.7%, p¼0.017), and a trend towards increased normal fertilization rates (73.8% vs 62.1%, p¼0.073) with increased injection time (2.5 vs 2.1 min, p¼0.001). No differences between HA-ICSI and PVP-ICSI were observed in (a) the proportion of good conventional morphology embryos (50% vs 53.1%, p¼0.712), (b) time-lapse qualitative measures (p>0.05) and (c) time-lapse quantitative measures (p>0.05). In conclusion, HA-ICSI improves fertilization outcomes although sperm injection takes longer to complete. Subsequent embryo development up to day 3 is not affected.