Immunoglobulin G N-glycan analysis by ultra-performance liquid chromatography

Document Type

Journal Article

Publication Title

Journal of Visualized Experiments : JoVE

Publisher

JoVE

School

School of Medical and Health Sciences

RAS ID

31463

Comments

Liu, D., Xu, X., Li, Y., Zhang, J., Zhang, X., Li, Q., ... & Wang, Y. (2020). Immunoglobulin G N-glycan analysis by ultra-performance liquid chromatography. Journal of Visualized Experiments: JoVE, 155, Article e60104. https://doi.org/10.3791/60104

Abstract

Glycomics is a new subspecialty in omics system research that offers significant potential in discovering next-generation biomarkers for disease susceptibility, drug target discovery, and precision medicine. Alternative IgG N-glycans have been reported in several common chronic diseases and suggested to have great potential in clinical applications (i.e., biomarkers for diagnosis and prediction of diseases). IgG N-glycans are widely characterized using the method of hydrophilic interaction chromatography (HILIC) ultra-performance liquid chromatography (UPLC). UPLC is a stable detection technology with good reproducibility and high relative quantitative accuracy. In addition, the structure of IgG N-glycan is clearly separated, and glycan composition and relative abundance in plasma are characterized.

DOI

10.3791/60104

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