The influence of ammonium nitrate, pH and indole butyric acid on root induction and survival in soil of micropropagated Eucalyptus globulus

Authors

I J. Bennett, Edith Cowan University
D A J McDavid
J A McComb

Document Type

Journal Article

Publisher

Springer

Faculty

Faculty of Computing, Health and Science

School

School of Natural Sciences / Centre for Ecosystem Management

RAS ID

8604

Comments

Bennett, I. J., McDavid, D. A. J., & McComb, J. A. (2003). The influence of ammonium nitrate, pH and indole butyric acid on root induction and survival in soil of micropropagated Eucalyptus globulus. Biologia Plantarum, 47(3), 355-360. Available here

Abstract

Rooting of Eucalyptus globulus shoots was influenced by the concentration of the indole butyric acid (IBA) and NH₄ ⁺ in the root-induction medium. Optimum plantlet vigor and survival were achieved using low concentrations (1 – 2.5 μM) of IBA and when NH₄NO₃ was removed. Removal of NH₄ ⁺ also had a significant effect on medium pH, its presence caused a decrease in pH as the culture period proceeded. When different nitrate compounds (excluding NH₄NO₃) were used as the nitrogen source, the medium pH was more stable and this was associated with higher root production. The higher root production, in association with appropriate IBA concentrations, produced plantlets with higher survival and better growth on transfer to soil.

DOI

10.1023/B:BIOP.0000023877.21262.a5