Document Type

Journal Article

Publisher

Mary Ann Liebert, Inc. Publishers

Faculty

Computing, Health and Science

School

Exercise, Biomedical & Health Science, Centre of Excellence for Alzheimer's Disease Research and Care

RAS ID

10609

Comments

This article was originally published as: Newman, M., Wilson, L., Camp, E., Verdile, G. , Martins, R. N., & Lardelli, M. (2010). A Zebrafish melanophore model of Amyloid (beta) toxicity. Zebrafish, 7(2), 155-159. Original article available here. This is a copy of an article published in Zebrafish © 2010 [copyright Mary Ann Liebert, Inc.]; Zebrafish is available online at: http://www.liebertonline.com.

Abstract

Reliable animal models are required to facilitate the understanding of neurodegenerative pathways in Alzheimer’s disease. Animal models can also be employed to search for disease-modifying drugs. The embryos and larvae of zebrafish are particularly advantageous for this purpose. For Alzheimer’s disease, drugs that can ameliorate amyloidb (Ab) toxicity have therapeutic and=or prophylactic potential. We attempted to generate a zebrafish model of Ab toxicity that would be viable and fertile but have a highly visible pigmentation phenotype in larvae. The larvae could then be arrayed in microtiter plates to screen compound libraries for drugs acting to reduce Ab toxicity. We used the promoter of the zebrafish mitfa (nacre) gene to drive expression of the pathological 42 amino acid species of human Ab, Ab42, specifically in the highly visible melanophores (melanocytes) of transgenic zebrafish. However, the transgenic fish only showed an aberrant pigment phenotype in adults at the advanced age of 16 months. Nevertheless, our results show that alteration of zebrafish pigment pattern may be useful for analysis of toxic peptide action.

DOI

10.1089/zeb.2009.0628

 
COinS
 

Link to publisher version (DOI)

10.1089/zeb.2009.0628