Loss of Heterozygosity Analysis at selected Single Nucleotide Polymorphism Sites in the Intronic Regions of PAX7 via In Silico biology and Microsatelite Analysis

Authors

Marika Mitchell, Edith Cowan University
Diane Tabarini
Melanie Ziman, Edith Cowan UniversityFollow

Document Type

Journal Article

Publisher

American Journal of Science

Comments

Mitchell, M., Tabarini, D., & Ziman, M. (2006). Loss of Heterozygosity Analysis at selected Single Nucleotide Polymorphism Sites in the Intronic Regions of PAX7 via In Silico biology and Microsatelite Analysis. The Journal of American Science. 2 (4), 18 - 34. Available here

Abstract

Motivation. In this study, the eight intronic regions of PAX7 were scanned for Single Nucleotide Polymorphisms (SNPs), loss of heterozygosity via allele quantification, and tandem repeats that exist immediately before or after the genetic variations.

Method. Culmination of results based on the output of gel electrophoresis and Microsatelite analysis.

Results. The use of less invasive methods, In Silico Biology and Miccrosatellite analysis to identify Rhabbdomyosarcoma offering reduced discomfort to cancer patients.

Conclusions. Results of microsatellite analysis show a new method for detecting ERMS/ARMS in patients.

Access Rights

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