Document Type
Journal Article
Keywords
[RSTDPub], Association study, Chinese, CIDEA, Haplotype, Obesity, Polymorphismadult, allele, article, case control study, Chinese, Cidea gene, controlled study, female, gene, gene interaction, gene location, gene locus, genetic association, genetic variability, haplotype, human, major clinical study, male, matrix assisted laser desorption ionization time of flight mass spectrometry, obesity, risk assessment, risk factor, single nucleotide polymorphism, Apoptosis Regulatory Proteins, Asian Continental Ancestry Group, Case-Control Studies, Ethnic Groups, Female, Genetic Association Studies, Genetic Loci, Genetic Predisposition to Disease, Haplotypes, Humans, Logistic Models, Male, Middle Aged, Models, Genetic, Multifactor Dimensionality Reduction, Obesity, Polymorphism, Single Nucleotide
Publisher
Springer
Faculty
Faculty of Health, Engineering and Science
School
School of Medical Sciences / Systems and Intervention Research Centre for Health
RAS ID
15863
Abstract
To investigate the association of tag-SNPs and haplotype structures of the CIDEA gene with obesity in a Han Chinese population. Five single nucleotide polymorphisms (SNPs) (rs1154588/V115F, rs4796955/SNP1, rs8092502/SNP2, rs12962340/SNP3 and rs7230480/SNP4) in the CIDEA gene were genotyped in a case-control study. Genotyping was performed using the sequenom matrixassisted laser desorption/ionization time-of-flight mass spectrometry iPLEX platform. There were significant differences between the obese and control groups in genotype distributions of V115F (P>0.001), SNP1 (P = 0.006) and SNP2 (P = 0.005). Carriers of V115F-TT, SNP1-GG and SNP2-CC genotypes had a 2.84-fold (95 % CI 1.73-4.66), 2.19-fold (95 % CI 1.09-4.38) and 4.37-fold (95 % CI 1.21-15.08) increased risk for obesity, respectively. Haplotype analysis showed that GTTC (SNP1/ SNP2/V115F/SNP4) had 1.41-fold (95 % CI 1.02-1.95) increased risk for obesity; whereas, haplotype TTGC had 0.48-fold (95 % CI 0.24-0.96) decreased risk for obesity. Using the multifactor dimensionality reduction method, the best model including SNP1, SNP2, V115F and SNP4 polymorphisms was identified with a maximum testing accuracy to 59.32 % and a perfect cross-validation consistency of 10/10 (P = 0.011). Logistic analysis indicated that there was a significant interaction between SNP1 and V115F associated with obesity. Subjects having both genotypes of SNP1/GG and V115F/TT were more susceptible to obesity in the Han Chinese population (OR 2.66, 95 %: 1.22-5.80). Genotypes of V115F/TT, SNP1/ GG and SNP2/CC and haplotype GTTC of CIDEA gene were identified as risk factors for obesity in the Han Chinese population. The interaction between SNP1 and V115F could play a joint role in the development of obesity.
Access Rights
free_to_read
Comments
This is an Author's Accepted Manuscript of: Wu, J., Zhang, L., Zhang, J., Dai, Y., Bian, L., Song, M., Russell, A. C., & Wang, W. (2013). The genetic contribution of CIDEA polymorphisms, haplotypes and loci interaction to obesity in a Han Chinese population. Molecular Biology Reports: an international journal on molecular and cellular biology, 40(10), 5691-5699. The final publication is available at Springer via here