Document Type
Journal Article
Publisher
Hongkong Gangmao Group Company
School
School of Medical and Health Sciences
Abstract
Objective: To introduce a system using spiked seminal plasma for the QC of the MAR direct test, and to facilitate the determination of assay variability.
Methods: A simple quality control (QC) system for use in the direct MAR test was developed using samples prepared by adding serum to antibody-negative centrifuged seminal plasma to obtain optimal binding, and storing 0.4 mL aliquots at −20 °C in straws. The serum was either from vasectomised men (positive control) or an antibody-negative woman (negative control). QC samples were thawed and mixed 1:1 with donor semen and pre-incubated for 1 hr at 37 °C, and tested for sperm antibodies using the direct method of the MarScreen IgG kit (Fertility Technology Resources, USA). Two batches of controls were prepared and one of these was run on each day.
Results: The negative controls invariably gave binding of less than 5%, whereas the two positive controls had binding (mean ± sd) of 89.5% ± 6.2 % (coefficient of variation [CV] = 7.0%) and (97.2 ± 2.5) (CV = 2.6%).
Conclusions: In summary, spiked seminal plasma incubated with whole semen can be used as a QC sample in the direct MAR IgG test.
DOI
10.1016/S2305-0500(13)60116-5
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Comments
Yap, K., Cawley, T., & Matson, P. (2013). Internal quality control for the direct MAR IgG test: A simple and effective method using spiked seminal plasma. Asian Pacific Journal of Reproduction, 2(1), 49-51. Available here