Document Type
Journal Article
Publication Title
Journal of Agricultural and Food Chemistry
Publisher
ACS
School
School of Science
Funders
Edith Cowan University / InterGrain Pty Ltd., the Commonwealth Scientific and Industrial Research Organization (CSIRO) / Agriculture and Food / Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science
Abstract
The conversion of raw barley (Hordeum vulgare L.) to malt requires a process of controlled germination, where the grain is submerged in water to raise the moisture content to > 40%. The transmembrane proteins, aquaporins, influence water uptake during the initial stage of controlled germination, yet little is known of their involvement in malting. With the current focus on sustainability, understanding the mechanisms of water uptake and usage during the initial stages of malting has become vital in improving efficient malting practices. In this study, we used quantitative proteomics analysis of two malting barley genotypes demonstrating differing water-uptake phenotypes in the initial stages of malting. Our study quantified 19 transmembrane proteins from nine families, including seven distinct aquaporin isoforms, including the plasma intrinsic proteins (PIPs) PIP1;1, PIP2;1, and PIP2;4 and the tonoplast intrinsic proteins (TIPs) TIP1;1, TIP2;3, TIP3;1, and TIP3;2. Our findings suggest that the presence of TIP1;1, TIP3;1, and TIP3;2 in the mature barley grain proteome is essential for facilitating water uptake, influencing cell turgor and the formation of large central lytic vacuoles aiding storage reserve hydrolysis and endosperm modification efficiency. This study proposes that TIP3s mediate water uptake in malting barley grain, offering potential breeding targets for improving sustainable malting practices.
DOI
10.1021/acs.jafc.4c00884
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Comments
O'Lone, C. E., Juhász, A., Nye-Wood, M., Moody, D., Dunn, H., Ral, J. P., & Colgrave, M. P. (2024). Advancing sustainable malting practices: Aquaporins as potential breeding targets for improved water uptake during controlled germination of barley (hordeum vulgare l.). Journal of Agricultural and Food Chemistry, 72(17), 10149–10161. https://doi.org/10.1021/acs.jafc.4c00884