Title

Time-lapse videography of human oocytes following intracytoplasmic sperm injection: Events up to the first cleavage division

Document Type

Journal Article

Publisher

Elsevier

Faculty

Faculty of Health, Engineering and Science

School

School of Medical Sciences

RAS ID

17770

Comments

This article was originally published as: Liu Y., Chapple V., Roberts P., Ali J., Matson P. (2014). Time-lapse videography of human oocytes following intracytoplasmic sperm injection: Events up to the first cleavage division. Reproductive Biology, 14(4), 249-256. Original article available here

Abstract

A total of 341 fertilized and 37 unfertilized oocytes from 63 intracytoplasmic sperm injection (ICSI) treatment cycles were included for retrospective assessment using the Embryoscope™ time-lapse video system. The second polar body (pb2) extrusion occurred at 2.9 ± 0.1. h (range 0.70-10.15. h) relative to sperm injection. All oocytes reduced in size following sperm injection (p38 years compared to those (3.4 ± 0.2 vs. 2.8 ± 0.1, p 0.05 respectively). A shorter time of first cleavage division relative to either sperm injection or pb2 extrusion is associated with both top grade (AUC = 0.596 or 0.601, p = 0.006 or 0.004) and usable embryos (AUC = 0.638 or 0.632, p = 0.000 respectively) on Day 3. In summary, (i) pb2 of human oocytes extrudes at various times following sperm injection, (ii) the timing of pb2 extrusion is significantly delayed when female age >38 years, but not related to subsequent embryo development, (iii) all human oocytes reduce in size following sperm injection, (iv) completion of pb2 extrusion in the fertilized oocytes is a pivotal event in terminating shrinkage of the vitellus, and (v) time to first cleavage division either from sperm injection or pb2 extrusion is a significant predictive marker for embryo quality on Day 3.

DOI

10.1016/j.repbio.2014.08.003

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