Document Type

Journal Article

School

School of Medical and Health Sciences

RAS ID

20734

Comments

Originally published as: Peirce,K.L., Roberts, P., Ali, J., Matson, P.L.(2015). The preparation and culture of washed human sperm: A comparison of a suite of protein-free media with media containing human serum albumin, Asian Pacific Journal of Reproduction. 4(3) 222-227.

Abstract

Objective

To compare two suites of culture media (one with HSA and one protein-free (PF) supplemented with methylcellulose) for washing human sperm in IVF.

Methods

Semen samples (n = 41) underwent parallel density gradient preparation using PF or HSA-supplemented culture medium and subsequent yield, survival, morphology and motility were compared.

Results

The PF medium resulted in a significantly higher sperm yield (P < 0.0001), but similar sperm morphology (P = 0.822) and 24-h survival (P = 0.11). There was, however, a lower percentage of progressively motile sperm (P < 0.0001) and a higher proportion of sperm demonstrating non-progressive motility (P < 0.0001) in the PF medium when observed on a Makler Chamber, apparently an artefact as a similar sperm motility index was measured using a Sperm Quality Analyser (P = 0.83). Attachment of sperm in PF medium to the glass chamber reduced with time and any differences had disappeared after 6 min on the counting chamber.

Conclusion

These results support the use of PF media supplemented with methylcellulose as an alternative to HSA, although a modification to the manufacturer's protocol of 6-min pre-incubation before assessing sperm motility must be used. Further studies should investigate the function of such sperm prepared in PF medium.

DOI

10.1016/j.apjr.2015.06.006

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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