Human CAP10-like protein 46 kDa gene promotes malignancy in colorectal cancer

Author Identifier

wei wang

https://orcid.org/0000-0002-1430-1360

Document Type

Journal Article

Publication Title

OMICS: A Journal of Integrative Biology

Publisher

Mary Ann Liebert Inc.

School

School of Medical and Health Sciences

RAS ID

24961

Funders

National Health and Medical Research Council

Grant Number

NHMRC Number : 1112767

Comments

Fang, H., Chu, Q., Zhang, J., Wang, H., Yu, X., Ge, S., . . . Wang, W. (2017). Human CAP10-like protein 46 kDa gene promotes malignancy in colorectal cancer. OMICS: A Journal of Integrative Biology, 21(5), 266-274. https://doi.org/10.1089/omi.2017.0037

Abstract

Colon cancer patients have major unmet needs in terms of robust diagnostics and molecular biomarkers for personalized therapeutics. We have previously reported that human CAP10-like protein 46 kDa (hCLP46) is overexpressed in human acute myelogenous leukemia, T acute lymphoblastic leukemia, and leukemia cell lines. We extend this line of biomarker and diagnostic discovery research by investigating hCLP46 expression in colorectal cancer (CRC) tissues and examine the possibility of hCLP46 as a candidate biomarker for diagnosis and prognosis of CRC. Using a tissue microarray analysis approach, we found that hCLP46 is (1) overexpressed in 90 CRC tissues compared with 90 matched noncancerous tissues and (2) positively correlated with higher tumor-node-metastasis (TNM) stage, lymph node metastasis, and shorter survival time. Moreover, in vitro experiments demonstrated that downregulation of hCLP46 in CRC cells results in proliferation arrest and adhesion enhancement, while apoptosis is unchanged. Further transcriptome profile analysis corroborated that the adhesion pathway is related to hCLP46 downregulation. This report for the first time, to the best of our knowledge, demonstrates that hCLP46 promotes tumor malignancy in CRC cells. We suggest that hCLP46 is warranted for further research as a candidate biomarker for clinical phenotypes related to colon cancer.

DOI

10.1089/omi.2017.0037

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