A Dorso-Ventral Gradient of Pax6 in the Developing Retina Suggests a Role in Topographic Map Formation
Faculty of Computing, Health and Science
School of Exercise, Biomedical and Health Science
Expression of the transcription factor Pax6 was assessed immunohistochemically in embryonic chick retina during retino-tectal map formation. A low dorsal to high ventral gradient was found that correlated with expression of the axonal guidance cue EphB2. Furthermore, transfection of Pax6 into undifferentiated P19 cells up-regulated EphB2. The results raise the possibility that Pax6 is upstream of EphB2 and that its graded expression defines the dorso-ventral axis of the retino-tectal projection.