Document Type
Journal Article
Publication Title
ACS Omega
Volume
7
Issue
8
First Page
6737
Last Page
6759
Publisher
ACS
School
School of Medical and Health Sciences
RAS ID
44323
Funders
The National Health and Medical Research Council of Australia (2012–2016 Senior Research Fellowship #1020411, 2017-Principal Research Fellowship #1117602, ideas grant GNT1185213) / The Australian Translational Medicinal Chemistry Facility (ATMCF) / Monash Institute of Pharmaceutical Sciences (MIPS) / Australian Government’s National Collaborative Research Infrastructure Strategy (NCRIS) program via Therapeutic Innovation Australia (TIA)
Grant Number
NHMRC Numbers : 1020411, 1117602, GNT1185213
Grant Link
http://purl.org/au-research/grants/nhmrc/1020411 http://purl.org/au-research/grants/nhmrc/1117602
Abstract
Colonization of the gastrointestinal (GI) tract with pathogenic bacteria is an important risk factor for the development of certain potentially severe and life-threatening healthcare-associated infections, yet efforts to develop effective decolonization agents have been largely unsuccessful thus far. Herein, we report modification of the 1,2,4-oxadiazole class of antimicrobial compounds with poorly permeable functional groups in order to target bacterial pathogens within the GI tract. We have identified that the quaternary ammonium functionality of analogue 26a results in complete impermeability in Caco-2 cell monolayers while retaining activity against GI pathogens Clostridioides difficile and multidrug-resistant (MDR) Enterococcus faecium. Low compound recovery levels after oral administration in rats were observed, which suggests that the analogues may be susceptible to degradation or metabolism within the gut, highlighting a key area for optimization in future efforts. This study demonstrates that modified analogues of the 1,2,4-oxadiazole class may be potential leads for further development of colon-targeted antimicrobial agents.
DOI
10.1021/acsomega.1c06294
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Comments
Pitcher, N. P., Harjani, J. R., Zhao, Y., Jin, J., Knight, D. R., Li, L., ... & Baell, J. B. (2022). Development of 1, 2, 4-Oxadiazole Antimicrobial Agents to Treat Enteric Pathogens within the Gastrointestinal Tract. ACS omega, 7, 8, 6737–6759. https://doi.org/10.1021/acsomega.1c06294