Whole-genome sequencing links Clostridium (Clostridioides) difficile in a single hospital to diverse environmental sources in the community

Authors

Su-Chen Lim, Edith Cowan University
Deirdre Collins, Edith Cowan UniversityFollow
Korakrit Imwattana
Daniel R. Knight
Sicilia Perumalsamy
Natasza M.R. Hain-Saunders, Edith Cowan University
Papanin Putsathit, Edith Cowan UniversityFollow
David Speers
Thomas Riley, Edith Cowan UniversityFollow

Author Identifier

Su-Chen Lim

https://orcid.org/0000-0001-8983-6573

Deirdre A Collins

https://orcid.org/0000-0001-6754-9290

Natasza M.R. Hain-Saunders

https://orcid.org/0000-0002-4259-4340

Papanin Putsathit

https://orcid.org/0000-0001-9789-4239

Thomas V. Riley

https://orcid.org/0000-0002-1351-3740

Document Type

Journal Article

Publication Title

Journal of Applied Microbiology

Publisher

Wiley

School

School of Medical and Health Sciences

RAS ID

40562

Funders

WA Department of Health

National Health and Medical Research Council Early Career Fellowships

Grant Number

NHMRC Numbers : APP1156789, APP1138257

Grant Link

http://purl.org/au-research/grants/nhmrc/GNT1156789

http://purl.org/au-research/grants/nhmrc/1138257

Comments

This is an author's accepted manuscript of:

Lim, S. C., Collins, D. A., Imwattana, K., Knight, D. R., Perumalsamy, S., Hain‐Saunders, N. M., ... & Riley, T. V. (2022). Whole‐genome sequencing links Clostridium (Clostridioides) difficile in a single hospital to diverse environmental sources in the community. Journal of Applied Microbiology, 133(3), 1156-1168.

https://doi.org/10.1111/jam.15408

Abstract

Aims

To investigate if Clostridium (Clostridioides) difficile infection (CDI), traditionally thought of as hospital-acquired, can be genomically linked to hospital or community environmental sources, and to define possible importation routes from the community to the hospital.

Methods and Results

In 2019, C. difficile was isolated from 89/300 (29.7%) floor and 96/300 (32.0%) shoe sole samples at a tertiary hospital in Western Australia. Non-toxigenic C. difficile ribotype (RT) 010 predominated among floor (96.6%) and shoe sole (73.2%) isolates, while toxigenic RT 014/020 was most prevalent among contemporaneous clinical cases (33.0%) at the hospital. Whole-genome sequencing and high-resolution core genome single nucleotide polymorphism (cgSNP) analysis on C. difficile strains from hospital and community sources showed no clinical C. difficile RT 014/020 strains were genetically related, and evidence of frequent long-distance, multi-directional spread between humans, animals and the environment. In addition, cgSNP analysis of environmental RT 010 strains suggested transportation of C. difficile via shoe soles.

Conclusions

While C. difficile RT 014/020 appears to spread via routes outside the healthcare system, RT 010 displayed a pattern of possible importation from the community into the hospital.

Significance and Impact of Study

These findings suggest developing community-based infection prevention and control strategies could significantly lower rates of CDI in the hospital setting.

DOI

10.1111/jam.15408