Document Type

Journal Article

Publication Title

Cancers

Volume

13

Issue

24

Publisher

MDPI

School

School of Medical and Health Sciences / Centre for Precision Health / Centre of Excellence for Alzheimer's Disease Research and Care / Graduate Research

RAS ID

40565

Funders

Edith Cowan University

Cancer Council, Western Australia

Australia New Zealand Gynaecological Oncology Group

Comments

Asante, D. B., Morici, M., Mohan, G. R. K. A., Acheampong, E., Spencer, I., Lin, W., . . . Gray, E. S. (2021). Multi-marker immunofluorescent staining and pd-l1 detection on circulating tumour cells from ovarian cancer patients. Cancers, 13(24), article 6225.

https://doi.org/10.3390/cancers13246225

Abstract

Detection of ovarian cancer (OC) circulating tumour cells (CTCs) is primarily based on targeting epithelial markers, thus failing to detect mesenchymal tumour cells. More importantly, the immune checkpoint inhibitor marker PD-L1 has not been demonstrated on CTCs from OC patients. An antibody staining protocol was developed and tested using SKOV-3 and OVCA432 OC cell lines. We targeted epithelial (cytokeratin (CK) and EpCAM), mesenchymal (vimentin), and OC-specific (PAX8) markers for detection of CTCs, and CD45/16 and CD31 were used for the exclusion of white blood and vascular endothelial cells, respectively. PD-L1 was used for CTC characterisation. CTCs were enriched using the Parsortix™ system from 16 OC patients. Results revealed the presence of CTCs in 10 (63%) cases. CTCs were heterogeneous, with 113/157 (72%) cells positive for CK/EpCAM (epithelial marker), 58/157 (37%) positive for vimentin (mesenchymal marker), and 17/157 (11%) for both (hybrid). PAX8 was only found in 11/157 (7%) CTCs. In addition, 62/157 (39%) CTCs were positive for PD-L1. Positivity for PD-L1 was significantly associated with the hybrid phenotype when compared with the epithelial (p = 0.007) and mesenchymal (p = 0.0009) expressing CTCs. Characterisation of CTC phenotypes in relation to clinical outcomes is needed to provide insight into the role that epithelial to mesenchymal plasticity plays in OC and its relationship with PD-L1.

DOI

10.3390/cancers13246225

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Research Themes

Health

Priority Areas

Multidisciplinary biological approaches to personalised disease diagnosis, prognosis and management

Included in

Diseases Commons

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