Document Type

Journal Article

Publication Title



Public Library of Science

Place of Publication

United States


School of Medical and Health Sciences




Originally published as: Jamnick, N. A., Botella, J., Pyne, D. B., & Bishop, D. J. (2018). Manipulating graded exercise test variables affects the validity of the lactate threshold and V˙ O2peak. PloS one, 13(7), e0199794. Original article available here.


Background To determine the validity of the lactate threshold (LT) and maximal oxygen uptake (V_ O2max) determined during graded exercise test (GXT) of different durations and using different LT calculations. Trained male cyclists (n = 17) completed five GXTs of varying stage length (1, 3, 4, 7 and 10 min) to establish the LT, and a series of 30-min constant power bouts to establish the maximal lactate steady state (MLSS). V_ O2 was assessed during each GXT and a subsequent verification exhaustive bout (VEB), and 14 different LTs were calculated from four of the GXTs (3, 4, 7 and 10 min)—yielding a total 56 LTs. Agreement was assessed between the highest V_ O2 measured during each GXT (V_ O2peak) as well as between each LT and MLSS. V_ O2peak and LT data were analysed using mean difference (MD) and intraclass correlation (ICC). Results The V_ O2peak value from GXT1 was 61.0 ± 5.3 and the peak power 420 ± 55 W (mean ± SD). The power at the MLSS was 264 ± 39 W. V_ O2peak from GXT3, 4, 7,10 underestimated V_ O2peak by ~1–5 Many of the traditional LT methods were not valid and a newly developed Modified Dmax method derived from GXT4 provided the most valid estimate of the MLSS (MD = 1.1 W; ICC = 0.96). Conclusion The data highlight how GXT protocol design and data analysis influence the determination of both V_ O2peak and LT. It is also apparent that V_ O2max and LT cannot be determined in a single GXT, even with the inclusion of a VEB.



Access Rights