Carlos Aya-Bonilla, Edith Cowan UniversityFollow
Elin S. Gray, Edith Cowan UniversityFollow
James B. Freeman, Edith Cowan UniversityFollow
Pauline Zaenker, Edith Cowan UniversityFollow
Anna L. Reid, Edith Cowan UniversityFollow
Muhammad A. Khattak
Markus H. Frank, Edith Cowan UniversityFollow
Mel Ziman, Edith Cowan UniversityFollow
Carlos Aya-Bonilla Orcid: https://orcid.org/0000-0001-6122-783X Elin Gray Orcid: https://orcid.org/0000-0002-8613-3570 James Freeman Orcid: https://orcid.org/0000-0001-8065-8416 Pauline Zaenker Orcid: https://orcid.org/0000-0002-0807-1968 Mel Ziman Orcid: https://orcid.org/0000-0001-7527-3538
School of Medical and Health Sciences
NHMRC Number : 1013349
Cutaneous melanoma circulating tumour cells (CTCs) are phenotypically and molecularly heterogeneous. We profiled the gene expression of CTC subpopulations immunomagnetic-captured by targeting either the melanoma-associated marker, MCSP, or the melanoma-initiating marker, ABCB5. Firstly, the expression of a subset of melanoma genes was investigated by RT-PCR in MCSP-enriched and ABCB5-enriched CTCs isolated from a total of 59 blood draws from 39 melanoma cases. Of these, 6 MCSP-and 6 ABCB5-enriched CTC fractions were further analysed using a genome-wide gene expression microarray. The transcriptional programs of both CTC subtypes included cell survival maintenance, cell proliferation, and migration pathways. ABCB5-enriched CTCs were specifically characterised by up-regulation of genes involved in epithelial to mesenchymal transition (EMT), suggesting an invasive phenotype. These findings underscore the presence of at least two distinct melanoma CTC subpopulations with distinct transcriptional programs, which may have distinct roles in disease progression and response to therapy. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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Prevention, detection and management of cancer and other chronic diseases