Document Type

Journal Article

Publication Title

Applications in Plant Sciences

Publisher

Wiley

School

Centre for Ecosystem Management

RAS ID

29808

Funders

Edith Cowan University Industry Collaboration Grant

Department of Biodiversity, Conservation and Attractions of Western Australia (G1002531)

Comments

Originally published as: Delnevo, N., Piotti, A., van Etten, E. J., Stock, W. D., & Byrne, M. (2019). Isolation, characterization, and cross‐amplification of 20 microsatellite markers for Conospermum undulatum (Proteaceae). Applications in Plant Sciences, 7(8), Article e11283. Original publication available here

Abstract

PREMISE: Recent habitat fragmentation is posing a risk to the wavy‐leaved smokebush, Conospermum undulatum (Proteaceae), a rare plant species endemic to southwestern Western Australia. Microsatellite markers are required to characterize the genetic diversity and structure of the species for conservation purposes and to facilitate ecological studies.

METHODS AND RESULTS: Illumina MiSeq high‐throughput sequencing was used to develop 20 novel microsatellite markers for C. undulatum. Polymorphism at each locus was assessed using 72 individuals from three natural populations. Nineteen markers were polymorphic, with the number of alleles per locus ranging from two to 21, and observed and expected heterozygosity ranging from 0.000 to 1.000 and 0.117 to 0.919, respectively. All markers successfully amplified in three congeneric species (C. stoechadis, C. canaliculatum and C. triplinervium).

CONCLUSIONS: The microsatellite markers will be useful for revealing patterns of genetic diversity, dispersal dynamics, and hybridization events for C. undulatum to inform future conservation efforts.

DOI

10.1002/aps3.11283

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

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