Document Type

Journal Article

Publication Title

Neural Regeneration Research

Publisher

Wolters Kluwer

School

Centre of Excellence for Alzheimer’s Disease Research and Care / School of Medical and Health Sciences

RAS ID

31551

Funders

National Health and Medical Research Council, NHMRC

Grant Number

NHMRC Number : 1107109

Comments

Bharadwaj, P., & Martins, R. (2020). A rapid absorbance-based growth assay to screen the toxicity of oligomer Aβ42 and protect against cell death in yeast. Neural Regeneration Research, 15(10), Article 1931. https://doi.org/10.4103/1673-5374.280318

Abstract

Multiple lines of evidence show that soluble oligomer forms of amyloid β protein (Aβ42) are the most neurotoxic species in the brain and correlates with the degree of neuronal loss and cognitive deficit in Alzheimer's disease. Although many studies have used mammalian cells to investigate oligomer Aβ42 toxicity, the use of more simple eukaryotic cellular systems offers advantages for large-scale screening studies. We have previously established and validated budding yeast, Saccharomyces cerevisiae to be a simple and a robust model to study the toxicity of Aβ. Using colony counting based methods, oligomeric Aβ42 was shown to induce dose-dependent cell death in yeast. We have adapted this method for high throughput screening by developing an absorbance-based growth assay. We further validated the assay with treatments previously shown to protect oligomer Aβ42 induced cell death in mammalian and yeast cells. This assay offers a platform for studying underlying mechanisms of oligomer Aβ42 induced cell death using gene deletion/overexpression libraries and developing novel agents that alleviate Aβ42 induced cell death. © 2020 Neural Regeneration Research. All rights reserved.

DOI

10.4103/1673-5374.280318

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

Research Themes

Health

Priority Areas

Neuroscience and neurorehabilitation

Share

 
COinS