Author Identifier

Prashant Bharadwaj

Orcid: https://orcid.org/0000-0003-4361-9906

Giuseppe Verdile

Orcid: https://orcid.org/0000-0003-2475-0124

Ralph Martins

Orcid: https://orcid.org/0000-0002-4828-9363

Document Type

Journal Article

Publication Title

Nature Scientific Reports

Publisher

Springer Nature

School

Centre of Excellence for Alzheimer’s disease Research and Care / School of Medical and Health Sciences

RAS ID

31904

Funders

Edith Cowan University - Open Access Support Scheme

NHMRC Dementia research development fellowship

Grant Number

NHMRC Number : 1107109

Grant Link

http://purl.org/au-research/grants/nhmrc/1107109

Comments

Bharadwaj, P., Solomon, T., Sahoo, B.R., Ignasiak, K., Gaskin, S., Rowles, J., ... & Martins, R. N. (2020). Amylin and beta amyloid proteins interact to form amorphous heterocomplexes with enhanced toxicity in neuronal cells. Scientific Reports, 10 Article 10356. https://doi.org/10.1038/s41598-020-66602-9

Abstract

Human pancreatic islet amyloid polypeptide (hIAPP) and beta amyloid (Aβ) can accumulate in Type 2 diabetes (T2D) and Alzheimer’s disease (AD) brains and evidence suggests that interaction between the two amyloidogenic proteins can lead to the formation of heterocomplex aggregates. However, the structure and consequences of the formation of these complexes remains to be determined. The main objective of this study was to characterise the different types and morphology of Aβ-hIAPP heterocomplexes and determine if formation of such complexes exacerbate neurotoxicity. We demonstrate that hIAPP promotes Aβ oligomerization and formation of small oligomer and large aggregate heterocomplexes. Co-oligomerized Aβ42-hIAPP mixtures displayed distinct amorphous structures and a 3-fold increase in neuronal cell death as compared to Aβ and hIAPP alone. However, in contrast to hIAPP, non-amyloidogenic rat amylin (rIAPP) reduced oligomer Aβ-mediated neuronal cell death. rIAPP exhibited reductions in Aβ induced neuronal cell death that was independent of its ability to interact with Aβ and form heterocomplexes; suggesting mediation by other pathways. Our findings reveal distinct effects of IAPP peptides in modulating Aβ aggregation and toxicity and provide new insight into the potential pathogenic effects of Aβ-IAPP hetero-oligomerization and development of IAPP based therapies for AD and T2D.

DOI

10.1038/s41598-020-66602-9

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Share

 
COinS