Publication Date


Document Type


School or Research Centre

School of Science / Centre for Marine Ecosystem Research (CMER)


This work was supported by the School of Science, Edith Cowan University (ECU), The Department of Biodiversity, Conservation and Attractions, Western Australia, and the Collaborative Research Network (CRN) comprising ECU and The University of Western Australia.


The database compiles data (used in Tarquinio et al. 2018, ISME Journal, accepted for publication) obtained from nitrogen stable isotope analysis (IRMS) and Nanoscale secondary ion mass spectrometry (NanoSIMS) of seagrass (Posidonia sinuosa) leaves and associated microorganisms. Row data (IRMS) are presented for bulk tissue 15N enrichment of P. sinuosa leaves at different times of incubation (plotted as bar chart in the manuscript), as well as the enrichment detected through the drawing of regions of interest (ROI) from NanoSIMS image analysis and plotted as box plots in the manuscript.



Research Activity Title

The role of the seagrass leaf microbiome in assisting nitrogen uptake by the Western Australian seagrass, Posidonia sinuosa

Research Activity Description

Microorganisms play a key role in facilitating the cycling of several elements in coastal environments, including nitrogen (N). N is a key component for maintaining high seagrass productivity and is often the limiting nutrient in marine environments. Seagrasses harbour an abundant and diverse microbial community (the ‘microbiome’), however their ecological and functional roles related to the seagrass host are still poorly understood, in particular regarding N cycling. Microorganisms capable of mineralising dissolved organic nitrogen (DON) may play a pivotal role in enhancing N availability in coastal environments such as seagrass meadows. Thus, the overall aim of the project was to enhance current understanding of abundance and diversity of the microbial community associated with seagrass meadows and their ecological role, with specific focus on N cycling. This was achieved by using molecular techniques together with 15N-enrichment experiments and nanoscale imaging techniques.


We exposed P. sinuosa leaves, with and without microorganisms, to 50µM of 15N enriched amino acids –treatments- and 50µM of 14N amino acids –controls- over 12 hours. For each time point (0.5, 2, 6 and 12 hours) samples (n=3) were collected and washed briefly to remove any residual added 15N. Three ~1cm2 sections were cut six cm from the growing tip using a sterile scalpel. The sections were fixed in 2.5% glutaraldehyde in 0.1M phosphate buffered saline and kept at 4°C for NanoSIMS analyses. All remaining leaf material (for treatments and controls) was processed for IRMS analyses.

Start of data collection time period


End of data collection time period



CTL=Control (samples incubated with 14N)

TRT=Treatment (samples incubated with 15N)

Y=Biofilm present

N=Biofilm Absent

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Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License


Glenn A. Hyndes: g.hyndes


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