Simultaneous determination of key osmoregulants in halophytes using HPLC-ELSD
Document Type
Journal Article
Keywords
[RSTDPub], Betaine, Column liquid chromatography, ELSD, Mannitol, Osmoregulators, Prolineacetonitrile, alcohol, Atriplex cinerea extract, betaine, formic acid, mannitol, plant extract, proline, Rhagodia baccata extract, Stylosanthes guianensis extract, unclassified drug, article, evaporative light scattering detection, halophyte, high performance liquid chromatography, light scattering, limit of detection, nonhuman, osmoregulation, priority journal, reliability, sensitivity analysis
Publisher
Springer-Verlag
Faculty
Faculty of Health, Engineering and Science
School
School of Natural Sciences / Centre for Ecosystem Management
RAS ID
16153
Abstract
Osmoregulants are the substances produced by plants that assist in tolerating environmental stresses. Three commonly analysed osomoregulants include mannitol, betaine and proline. A simple, sensitive and rapid HPLC-ELSD method has been developed for the simultaneous analysis of these common osmoregulants in plant extracts. Osmoregulants were extracted using 80 % ethanol and separated on an NH2 column using 0.1 % formic acid and acetonitrile as the mobile phase. Retention time repeatability was 0.85, 1.50, and 0.93 % for mannitol, betaine and proline, respectively. The limit of detection (μmol) was 1.43 × 10-4, 7.81 × 10 -5 and 1.08 × 10-4 for mannitol, betaine and proline, respectively. The developed method was applied to three different plant extracts, Stylosanthes guianensis, Atriplex cinerea and Rhagodia baccata. A second method using a C18 column with 0.1 % heptafluorobutyric acid and acetonitrile as the mobile phase proved to be a useful complementary method for verifying tentative peak identifications.
Comments
Kalsoom, U. , Boyce, M. C., Bennett, I. J., & Veraplakorn, V. (2013). Simultaneous determination of key osmoregulants in halophytes using HPLC-ELSD. Chromatographia, 76(17-18), 1125-1130. Available here