Evaluation of protein extraction methods for in-depth proteome analysis of narrow-leafed lupin (Lupinus angustifolius) seeds
Document Type
Journal Article
Publication Title
Food chemistry
Volume
367
First Page
130722
PubMed ID
34375893
Publisher
Elsevier
School
School of Science
RAS ID
36352
Funders
Australian Government research training program (RTP) scholarship and Commonwealth Scientific and Industrial Research Organisation top-up postgraduate scholarship
Abstract
Lupin is slated as a potential contributor towards future food security. Lupin possesses several nutritional and nutraceutical attributes, many linked to seed proteins. For in-depth characterisation of the lupin proteome, liquid chromatography-tandem mass spectrometry was used to evaluate four protein extraction procedures. The proteomes of three narrow-leafed lupin were qualitatively evaluated using protein/peptide identifications and further quantitatively assessed by data-independent proteome measurement. Each extraction buffer led to unique protein identifications; altogether yielding 2,760 protein identifications from lupin varieties. The analysis of protein abundance data highlighted distinct differences between Tris-HCl and urea extracted proteomes, while also revealing variation amongst the cultivar proteomes with the wild accession (P27255) distinctly different from the domesticated cultivars (Tanjil, Unicrop). The extraction buffer used influenced the proteome coverage, downstream functional annotation results and consequently the biological interpretation demonstrating the need to optimise and understand the impact of protein extraction conditions.
DOI
10.1016/j.foodchem.2021.130722
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Comments
Tahmasian, A., Broadbent, J. A., Juhász, A., Nye-Wood, M., Le, T. T., Bose, U., & Colgrave, M. L. (2022). Evaluation of protein extraction methods for in-depth proteome analysis of narrow-leafed lupin (Lupinus angustifolius) seeds. Food Chemistry, 367, 130722. https://doi.org/10.1016/j.foodchem.2021.130722