Title

Evaluation of protein extraction methods for in-depth proteome analysis of narrow-leafed lupin (Lupinus angustifolius) seeds

Document Type

Journal Article

Publication Title

Food chemistry

Volume

367

First Page

130722

PubMed ID

34375893

Publisher

Elsevier

School

School of Science

RAS ID

36352

Funders

Australian Government research training program (RTP) scholarship and Commonwealth Scientific and Industrial Research Organisation top-up postgraduate scholarship

Comments

Tahmasian, A., Broadbent, J. A., Juhász, A., Nye-Wood, M., Le, T. T., Bose, U., & Colgrave, M. L. (2022). Evaluation of protein extraction methods for in-depth proteome analysis of narrow-leafed lupin (Lupinus angustifolius) seeds. Food Chemistry, 367, 130722. https://doi.org/10.1016/j.foodchem.2021.130722

Abstract

Lupin is slated as a potential contributor towards future food security. Lupin possesses several nutritional and nutraceutical attributes, many linked to seed proteins. For in-depth characterisation of the lupin proteome, liquid chromatography-tandem mass spectrometry was used to evaluate four protein extraction procedures. The proteomes of three narrow-leafed lupin were qualitatively evaluated using protein/peptide identifications and further quantitatively assessed by data-independent proteome measurement. Each extraction buffer led to unique protein identifications; altogether yielding 2,760 protein identifications from lupin varieties. The analysis of protein abundance data highlighted distinct differences between Tris-HCl and urea extracted proteomes, while also revealing variation amongst the cultivar proteomes with the wild accession (P27255) distinctly different from the domesticated cultivars (Tanjil, Unicrop). The extraction buffer used influenced the proteome coverage, downstream functional annotation results and consequently the biological interpretation demonstrating the need to optimise and understand the impact of protein extraction conditions.

DOI

10.1016/j.foodchem.2021.130722

Research Themes

Health

Priority Areas

Prevention, detection and management of cancer and other chronic diseases

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