Document Type
Journal Article
Publication Title
Food Chemistry
Volume
417
Publisher
Elsevier
School
School of Science
Funders
Research Plus CERC postdoctoral fellowship by CSIRO, Australia
Abstract
Accidental milk cross-contamination is one of the most common causes for costly food recalls. Yet, quantifying trace-levels of allergen is time-consuming and current methods are not adapted for routine analyses making quality control for trace-level allergen content impractical. This perpetuates voluntary “may-contain” statements that are unhelpful for people suffering from food allergies. Here, we developed a rapid LC-MS method enabling milk allergen quantification by comparing all tryptic-peptides of major milk allergens. The bovine-specific αS-2 casein peptide and allergen-epitope NAVPITPTLNR provided excellent performance in sensitivity (LOD 1 mg.kg − 1; LOQ 2 mg.kg − 1) across various dairy products, good recovery rates in baked croissants (77 % with a 10 % inter-day RSD) and a linear range of 2 – 2,000 mg.kg − 1. The method can be used for routine determination of trace-contamination with bovine milk allergen and the adulteration of high-value caprine dairy products with lower-value bovine milk products, protecting consumer trust and the growing population suffering from food allergies.
DOI
10.1016/j.foodchem.2023.135885
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Comments
Nelis, J. L., Dawson, A. L., Bose, U., Anderson, A., Colgrave, M. L., & Broadbent, J. A. (2023). Safe food through better labelling: A robust method for the rapid determination of caprine and bovine milk allergens. Food Chemistry, 417, Article 135885.
https://doi.org/10.1016/j.foodchem.2023.135885