Document Type
Journal Article
Publication Title
Epigenetics
Volume
18
Issue
1
First Page
2211361
PubMed ID
37233989
Publisher
Taylor & Francis
School
School of Medical and Health Sciences
RAS ID
60153
Funders
National Health and Medical Research Council / Edith Cowan University
Further funding information https://doi.org/10.1080/15592294.2023.2211361
Grant Number
NHMRC Numbers : 572613, 403981, 1059711, 1053384, 2001203, 353514, 403981, 572613, 1059711,
Grant Link
http://purl.org/au-research/grants/nhmrc/572613
http://purl.org/au-research/grants/nhmrc/1059711
http://purl.org/au-research/grants/nhmrc/1053384
http://purl.org/au-research/grants/nhmrc/2001203
http://purl.org/au-research/grants/nhmrc/572613
http://purl.org/au-research/grants/nhmrc/1059711
Abstract
BACKGROUND: Dietary intake of antioxidants such as vitamins C and E protect against oxidative stress, and may also be associated with altered DNA methylation patterns. METHODS: We meta-analysed epigenome-wide association study (EWAS) results from 11,866 participants across eight population-based cohorts to evaluate the association between self-reported dietary and supplemental intake of vitamins C and E with DNA methylation. EWAS were adjusted for age, sex, BMI, caloric intake, blood cell type proportion, smoking status, alcohol consumption, and technical covariates. Significant results of the meta-analysis were subsequently evaluated in gene set enrichment analysis (GSEA) and expression quantitative trait methylation (eQTM) analysis. RESULTS: In meta-analysis, methylation at 4,656 CpG sites was significantly associated with vitamin C intake at FDR ≤ 0.05. The most significant CpG sites associated with vitamin C (at FDR ≤ 0.01) were enriched for pathways associated with systems development and cell signalling in GSEA, and were associated with downstream expression of genes enriched in the immune response in eQTM analysis. Furthermore, methylation at 160 CpG sites was significantly associated with vitamin E intake at FDR ≤ 0.05, but GSEA and eQTM analysis of the top most significant CpG sites associated with vitamin E did not identify significant enrichment of any biological pathways investigated. CONCLUSIONS: We identified significant associations of many CpG sites with vitamin C and E intake, and our results suggest that vitamin C intake may be associated with systems development and the immune response.
DOI
10.1080/15592294.2023.2211361
Creative Commons License
This work is licensed under a
Creative Commons Public Domain Mark 1.0.
Comments
Keshawarz, A., Joehanes, R., Ma, J., Lee, G. Y., Costeira, R., Tsai, P. C., . . . Levy, D. (2023). Dietary and supplemental intake of vitamins C and E is associated with altered DNA methylation in an epigenome-wide association study meta-analysis. Epigenetics, 18(1), article 2211361.
https://doi.org/10.1080/15592294.2023.2211361