Document Type
Journal Article
Publication Title
Biophysics Reports
Volume
9
Issue
1
First Page
15
Last Page
25
Publisher
Biophysical Society of China
School
Centre for Precision Health
RAS ID
60302
Funders
National Key Research and Development Program of China / Beijing Municipal Natural Science Foundation / National Natural Science Foundation of China / CAS Interdisciplinary Innovation Team
Abstract
3D genomics mainly focuses on the 3D position of single genes at the cell level, while spatial genomics focuses more on the tissue level. In this exciting new era of 3D/spatial genomics, half-century old FISH and its derivative methods, including Tn5-FISH, play important roles. In this review, we introduce the Tn5-FISH we developed recently, and present six different applications published by our collaborators and us, based on (Tn5-)FISH, which can be either general BAC clone-based FISH or Tn5-FISH. In these interesting cases, (Tn5-)FISH demonstrated its vigorous ability of targeting sub-chromosomal structures across different diseases and cell lines (leukemia, mESCs (mouse embryonic stem cells), and differentiation cell lines). Serving as an effective tool to image genomic structures at the kilobase level, Tn5-FISH holds great potential to detect chromosomal structures in a high-throughput manner, thus bringing the dawn for new discoveries in the great era of 3D/spatial genomics.
DOI
10.52601/bpr.2023.220025
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Comments
Yang, L., Yan, Y., Li, J. L., Zhou, C., Jin, J., Zhang, T., . . . Gao, J. (2023). (Tn5-)fish-based imaging in the era of 3D/spatial genomics. Biophysics Reports, 9(1), 15-25. http://www.biophysics-reports.org/en/article/doi/10.52601/bpr.2023.220025