Abstract
BCAM-positive basal limbal epithelial cells are an early transit-amplifying cell population (TAC) capable of holoclone formation and corneal epithelial differentiation. Here, we present a protocol for isolating BCAM-positive cells from human donor corneas by flow cytometry and cell sorting. We describe steps for cell dissection and dissociation, antibody staining, and flow cytometry. We then detail procedures for culturing the purified BCAM-positive and BCAM-negative cells for holoclone and cell sheet formation assays to study the factors that regulate corneal regeneration. For complete details on the use and execution of this protocol, please refer to Sasamoto et al.1
Document Type
Journal Article
Date of Publication
9-15-2023
Volume
4
Issue
3
Publication Title
STAR Protocols
Publisher
Elsevier
School
School of Medical and Health Sciences
RAS ID
62016
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Comments
Sasamoto, Y., Yeung, P. C., Tran, J., Frank, M. H., & Frank, N. Y. (2023). Protocol for isolating human BCAM-positive corneal progenitor cells by flow cytometry and cell sorting. STAR protocols, 4(3), 102503. https://doi.org/10.1016/j.xpro.2023.102503