Epigenome-wide meta-analysis reveals associations between dietary glycemic index and glycemic load and DNA methylation in children and adolescents of different body sizes

Document Type

Journal Article

Publication Title

Diabetes Care

Volume

46

Issue

11

First Page

2067

Last Page

2075

PubMed ID

37756535

Publisher

American Diabetes Association

School

Nutrition and Health Innovation Research Institute

RAS ID

64605

Funders

Joint Programming Initiative-A Healthy Diet for a Healthy Life / German Federal Ministry of Education and Research / Medical Research Council / Biotechnology and Biological Sciences Research Council / Instituto de Salud Carlos III / ZonMw

Comments

Ott, R., Stein, R., Hauta-alus, H. H., Ronkainen, J., Fernández-Barrés, S., Spielau, U., . . . Hummel, S. (2023). Epigenome-wide meta-analysis reveals associations between dietary glycemic index and glycemic load and DNA methylation in children and adolescents of different body sizes. Diabetes Care, 46(11), 2067-2075. https://doi.org/10.2337/dc23-0474

Abstract

Dietary glycemic index (GI) and glycemic load (GL) are associated with cardiome-tabolic health in children and adolescents, with potential distinct effects in people with increased BMI. DNA methylation (DNAm) may mediate these effects. Thus, we conducted meta-analyses of epigenome-wide association studies (EWAS) between dietary GI and GL and blood DNAm of children and adolescents. RESEARCH DESIGN AND METHODS We calculated dietary GI and GL and performed EWAS in children and adolescents (age range: 4.5–17 years) from six cohorts (N = 1,187). We performed stratified analyses of participants with normal weight (n = 801) or overweight or obesity (n = 386). We performed look-ups for the identified cytosine–phosphate–guanine (CpG) sites (false discovery rate [FDR] < 0.05) with tissue-specific gene expression of 832 blood and 223 subcutaneous adipose tissue samples from children and adolescents. RESULTS Dietary GL was positively associated with DNAm of cg20274553 (FDR < 0.05), anno-tated to WDR27. Several CpGs were identified in the normal-weight (GI: 85; GL: 17) and overweight or obese (GI: 136; GL: 298; FDR < 0.05) strata, and none overlapped between strata. In participants with overweight or obesity, identified CpGs were related to RNA expression of genes associated with impaired metabolism (e.g., FRAT1, CSF3). CONCLUSIONS We identified 537 associations between dietary GI and GL and blood DNAm, mainly in children and adolescents with overweight or obesity. High-GI and/or-GL diets may influence epigenetic gene regulation and thereby promote metabolic derangements in young people with increased BMI.

DOI

10.2337/dc23-0474

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free_to_read

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