Document Type
Journal Article
Publication Title
Frontiers in Microbiology
Volume
14
Publisher
Frontiers Media S.A.
School
Centre for Precision Health / School of Medical and Health Sciences
Funders
Xuzhou Medical University Open Research Grant / University of New South Wales
Abstract
Campylobacter concisus is an oral bacterium. Recent studies suggest that C. concisus may be involved in human gastric diseases. The mechanisms, however, by which C. concisus causes human gastric diseases have not been investigated. Here we examined the gastric epithelial pathogenicity of C. concisus using a cell culture model. Six C. concisus strains and the human gastric epithelial cell line AGS cells were used. IL-8 produced by AGS cells after incubation with C. concisus was measured using enzyme-linked immunosorbent assay (ELISA), and AGS cell apoptosis was determined by caspase 3/7 activities. The effects of C. concisus on actin arrangement in AGS cells was determined using fluorescence staining. The effects of C. concisus on global gene expression in AGS cells was determined by transcriptomic analysis and quantitative real-time PCR (qRT-PCR). The role of the upregulated CYP1A1 gene in gastric cancer survival was assessed using the Kaplan-Meier method. C. concisus induced production of IL-8 by AGS cells with strain variation. Significantly increased caspase 3/7 activities were observed in AGS cells incubated with C. concisus strains when compared to AGS cells without bacteria. C. concisus induced actin re-arrangement in AGS cells. C. concisus upregulated 30 genes in AGS cells and the upregulation of CYP1A1 gene was confirmed by qRT-PCR. The Kaplan-Meier analysis showed that upregulation of CYP1A1 gene is associated with worse survival in gastric cancer patients. Our findings suggest that C. concisus may play a role in gastric inflammation and the progression of gastric cancer. Further investigation in clinical studies is warranted.
DOI
10.3389/fmicb.2023.1289549
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Comments
Luk, C. Y. M., Lee, S. A., Naidovski, N., Liu, F., Tay, A. C. Y., Wang, L., . . . Zhang, L. (2024). Investigation of campylobacter concisus gastric epithelial pathogenicity using AGS cells. Frontiers in Microbiology, 14, article 1289549. https://doi.org/10.3389/fmicb.2023.1289549