Identification of circRNA biomarkers in osteosarcoma: An updated systematic review and meta-analysis
Document Type
Journal Article
Publication Title
Non-coding RNA Research
Volume
9
Issue
2
First Page
341
Last Page
349
Publisher
Elsevier
School
School of Medical and Health Sciences / Centre for Precision Health
RAS ID
65507
Funders
Provincial Science and Technology Special Fund of Guangdong, China
Abstract
Circular RNAs (circRNAs) play a crucial role in cancer development and progression. This study aimed to identify potential circRNA biomarkers for osteosarcoma. Articles published from January 2010 to September 2023 were searched across eight databases to compare circRNA expression profiles in osteosarcoma and control samples (human, animal and cell lines). Meta-analysis was conducted under a random effects model. Subgroup analysis of circRNAs in different samples and tissues was performed. Diagnostic value was evaluated using receiver operator characteristic curves. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis explored functions of circRNA host genes. A circRNA-miRNA-mRNA axis depicted the regulatory mechanism in osteosarcoma. Among 1356 circRNAs with differential expression were identified across 226 original studies, only 74 were reported in at least three published sub-studies. Meta-analysis identified 58 dysregulated circRNAs (52 upregulated and 6 downregulated). Eleven circRNAs consistently showed dysregulation in tissues and cell lines, with hsa_circ_0005721 showing potential as a circulating biomarker in osteosarcoma. Sensitivity analysis demonstrated 97 % consistency. The overall area under the curve was 0.87 (95 % CI, 0.83–0.89). GO and KEGG enrichment analyses revealed host gene involvement in cancer. The circRNA-miRNA-mRNA axis revealed the regulatory axis and interactions within osteosarcoma specifically. This study demonstrates circRNAs as potential diagnostic biomarkers for osteosarcoma. Consistently reported dysregulated circRNAs are potential biomarkers in osteosarcoma pathogenesis, with hsa_circ_0005721 as a potential circulating biomarker for diagnosis and treatment.
DOI
10.1016/j.ncrna.2024.01.007
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Comments
Zhou, C., Balmer, L., Song, M., Mahara, G., Wu, K., Wang, W., & Wang, H. (2024). Identification of circRNA biomarkers in osteosarcoma: An updated systematic review and meta-analysis. Non-coding RNA Research, 9(2), 341-349. https://doi.org/10.1016/j.ncrna.2024.01.007