Abstract
Barley is commonly used in malting and brewing, and spent grain is repurposed for other foods. Barley contains gluten proteins called hordeins that cause intestinal damage and disease symptoms if eaten by people with celiac disease and related conditions. While the mashing process in brewing can partially hydrolyze immunogenic epitopes in hordeins, the immunogenic epitope load between the starting malt and spent grain has not been investigated. Herein, we quantified hordeins in commercially available spent grain and from matching malt. Liquid chromatography-mass spectrometry (LC-MS) and sandwich and competitive R5 ELISAs were used for quantification, revealing a higher abundance of gluten proteins in the spent grain product compared with the input malt. Certain hordein subtypes were enriched while others were depleted, and overall protein content was higher in spent grain. This suggests that the mashing process selectively extracts nonprotein components, leaving protein and hordein content elevated in spent grain. The spent grain products tested were not safe for consumers with celiac disease.
Document Type
Journal Article
Date of Publication
3-6-2024
Volume
35
Issue
3
PubMed ID
38385353
Publication Title
Journal of the American Society for Mass Spectrometry
Publisher
ACS
School
School of Science
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
First Page
409
Last Page
412
Comments
Nye-Wood, M. G., & Colgrave M. L. (2024). LC-MS/MS reveals hordeins are enriched in brewers’ spent grain. Journal of the American Society for Mass Spectrometry, 35(3), 409-412. https://doi.org/10.1021/jasms.3c00451