Electrospun nanofibrous scaffolds as a platform to reduce melanoma tumour growth, recurrence, and promote post-resection wound repair
Abstract
Wound healing following skin tumour surgery still remains a major challenge. To address this issue, polysaccharide-loaded nanofibrous mats have been engineered as skin patches on the wound site to improve wound healing while simultaneously eliminating residual cancer cells which may cause cancer relapse. The marine derived polysaccharides kappa-carrageenan (KCG) and fucoidan (FUC) were blended with polydioxanone (PDX) nanofibers due to their inherent anti-cancer activity conferred by the sulphate groups as well as their immunomodulatory properties which can reduce inflammation resulting in accelerated wound healing. KCG and FUC were released sustainably from the blend nanofibers via the Korsmeyer-Peppas kinetics. MTT assays, live/dead staining and SEM images demonstrated the toxicity of KCG and FUC towards skin cancer MP 41 cells. In addition, MP 41 cells showed reduced metastatic potential when grown on KCG or FUC containing mats. Both KCG and FUC were non- cytotoxic to healthy L 929 fibroblast cells. In vivo studies on healthy Wistar rats confirmed the non-toxicity of the nanofibrous patches as well as their improved and scarless wound healing potential. In vivo studies on tumour xenograft model further showed a reduction of 7.15 % in tumour volume in only 4 days following application of the transdermal patch.
Document Type
Journal Article
Date of Publication
7-1-2024
Volume
161
Funding Information
RT Knits Ltd. / National Research Foundation (NRF) of South Africa
School
School of Medical and Health Sciences
Copyright
subscription content
Publisher
Elsevier
Recommended Citation
Goonoo, N., Gimié, F., Ait-Arsa, I., Ziman, M., Adeyemi, S. A., Ubanako, P., Ngema, L. M., Choonara, Y. E., & Bhaw-Luximon, A. (2024). Electrospun nanofibrous scaffolds as a platform to reduce melanoma tumour growth, recurrence, and promote post-resection wound repair. DOI: https://doi.org/10.1016/j.bioadv.2024.213870
Comments
Goonoo, N., Gimié, F., Ait-Arsa, I., Ziman, M., Adeyemi, S. A., Ubanako, P., . . . Bhaw-Luximon, A. (2024). Electrospun nanofibrous scaffolds as a platform to reduce melanoma tumour growth, recurrence, and promote post-resection wound repair. Biomaterials Advances, 161, article 213870. https://doi.org/10.1016/j.bioadv.2024.213870