Document Type

Journal Article

Publication Title

Pediatric Allergy and Immunology

Volume

35

Issue

9

PubMed ID

39221598

Publisher

Wiley

School

School of Medical and Health Sciences

Funders

WA Child Research Fund (RA/1/3027/386) / Family Larsson-Rosenquist Foundation / National Health and Medical Research Council / Telethon-Perth Children's Hospital Research Fund / Career Development Fellowship / Telethon Kids Institute Ascend Fellowship / Government of Australia / Paul Ramsay Foundation / Joondalup Health Campus

Grant Number

NHMRC Number : 1099480, 1144544

Comments

Divakara, N., Dempsey, Z., Saraswati, C., Garssen, J., Silva, D., Keelan, J. A., ... & Macchiaverni, P. (2024). Effect of maternal prebiotic supplementation on human milk immunological composition: Insights from the SYMBA study. Pediatric Allergy and Immunology, 35(9). https://doi.org/10.1111/pai.14226

Abstract

Background: Immunomodulatory proteins in human milk (HM) can shape infant immune development. However, strategies to modulate their levels are currently unknown. This study investigated whether maternal prebiotic supplementation alters the levels of immunomodulatory proteins in HM. Methods: The study was nested within the SYMBA double-blind randomized controlled trial (ACTRN12615001075572), which investigated the effects of maternal prebiotic (short-chain galacto-oligosaccharides/long-chain fructo-oligosaccharides) supplementation from <21 weeks gestation during pregnancy until 6 months postnatal during lactation on child allergic disease risk. Mother–child dyads receiving prebiotics (n = 46) or placebo (n = 54) were included in this study. We measured the levels of 24 immunomodulatory proteins in HM collected at 2, 4, and 6 months. Results: Cluster analysis showed that the overall immunomodulatory protein composition of milk samples from both groups was similar. At 2 months, HM of prebiotic-supplemented women had decreased levels of TGF-β1 and TSLP (95% CI: −17.4 [−29.68, −2.28] and −57.32 [−94.22, −4.7] respectively) and increased levels of sCD14 (95% CI: 1.81 [0.17, 3.71]), when compared to the placebo group. At 4 months, IgG1 was lower in the prebiotic group (95% CI: −1.55 [−3.55, −0.12]) compared to placebo group. Conclusion: This exploratory study shows that prebiotic consumption by lactating mothers selectively alters specific immunomodulatory proteins in HM. This finding is crucial for understanding how prebiotic dietary recommendations for pregnant and lactating women can modify the immune properties of HM and potentially influence infant health outcomes through immune support from breastfeeding.

DOI

10.1111/pai.14226

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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