Evaluation of alkaloid levels in commercial and wild genotypes of narrow-leafed lupin

Authors

Tawfeek Khedr, Edith Cowan UniversityFollow
Ling Ling Gao
Lars G. Kamphuis
Utpal Bose, Edith Cowan UniversityFollow
Angéla Juhász, Edith Cowan UniversityFollow
Michelle L. Colgrave, Edith Cowan UniversityFollow

Document Type

Journal Article

Publication Title

Journal of Food Composition and Analysis

Volume

135

Publisher

Elsevier

School

Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science / School of Science

Funders

Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science / Australian Government Research Training Program

Grant Number

ARC Number : CE200100012

Comments

Khedr, T., Gao, L. L., Kamphuis, L. G., Bose, U., Juhász, A., & Colgrave, M. L. (2024). Evaluation of alkaloid levels in commercial and wild genotypes of narrow-leafed lupin. Journal of Food Composition and Analysis, 135. https://doi.org/10.1016/j.jfca.2024.106600

Abstract

Lupins are a promising source of protein that can complement soybean-based proteins in food and animal feed. Narrow-leafed lupin (Lupinus angustifolius) is the predominant lupin species cultivated in Australia and Europe. Consumption of lupin products can lead to toxicity in both humans and animals, primarily due to the presence of naturally occurring alkaloid compounds. The aim of the present research was to examine the alkaloid content of commercial narrow-leafed lupin grown in Australia and compare this to wild accessions. Nineteen accessions of narrow-leafed lupin were grown in a growth chamber with a day/night cycle of 18 h of light and 6 h of darkness. An LC-MS/MS-based method was deployed to detect and quantify the main alkaloids in lupins: angustifoline, 13α-hydroxylupanine, (+) lupanine, sparteine, (-) lupinine, isolupanine, and gramine. The alkaloid content in narrow-leafed lupin exhibited a wide variation among different genotypes. Sweet varieties such as Geebung had remarkably low alkaloid content, below 10 mg/kg. Bitter accessions such as P22660 displayed considerably higher alkaloid levels, as high as 20,000 mg/kg. The level of variation of alkaloids observed within lupin grain from different plant replicates of the same cultivars was significantly greater in sweet phenotypes (SD > 100 %) compared to bitter cultivars (SD < 20 %).

DOI

10.1016/j.jfca.2024.106600

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