Author Identifier
J. M. Hodgson: https://orcid.org/0000-0001-6184-7764
C. P. Bondonno: https://orcid.org/0000-0001-8509-439X
Document Type
Journal Article
Publication Title
Analytical Methods
Volume
16
Issue
46
First Page
8001
Last Page
8009
Publisher
Royal Society of Chemistry
School
Nutrition and Health Innovation Research Institute / School of Medical and Health Sciences
Funders
Western Australian Future Health Research and Innovation Fund / Department of Health, Government of Western Australia (WANMA/EL2022/3, WANMA2023Ideas/3) / RPH Research Foundation (SB-Plus 196/2022)
Abstract
N-Nitrosamines, carcinogenic compounds present in dietary and environmental sources and formed endogenously, are believed to be linked with the presence of nitrate and nitrite, both within dietary sources and after intake. To fully evaluate this potential threat to human health, an accurate analytical method to measure N-nitrosamines in biological matrices is necessary. We report a simple, fast, selective mass spectrometry method to detect N-nitrosamines in human urine. Analysis of seven N-nitrosamines, N-nitrosodimethylamine (NDMA), N-nitrosomethylethylamine (NMEA), N-nitrosodiethylamine (NDEA), N-nitrosopiperdine (NPIP), N-nitrosopyrrolidine (NPYR), N-nitrosodi-N-propylamine (NDPA) and N-nitrosodi-N-butylamine (NDBA) in urine was quantitated using Ultra High-Pressure Liquid Chromatography-tandem Mass spectrometry (UHPLC-MS/MS). A Sorbent supported Liquid Extraction (SLE) method was employed to extract N-nitrosamines from 24 hour collected human urine samples. The percent recovery varied between 74.3 to 110 and the limit of detection and limit of quantification ranged from 0.1 to 0.85 ng mL−1 and 0.22 to 2.06 ng mL−1 respectively. Precision for inter-day and intra-day assay yielded a % coefficient of variation between 4-10% for all measured compounds in urine. Linear regression analysis of calibration curves for N-nitrosamines measured in urine in the concentration range 0.4-12.8 ng mL−1 gave correlation coefficients, R2 0.9874-0.9962. Urinary excretion of N-nitrosamines measured in ten healthy subjects resulted in detection of most of the N-nitrosamines including NDMA, NDEA, NPYR, NDPA and NDBA by this method.
DOI
10.1039/d4ay01870b
Creative Commons License
This work is licensed under a Creative Commons Attribution-No Derivative Works 4.0 License.
Comments
Reproduced from Shinde, S., Croft, K. D., Hodgson, J. M., & Bondonno, C. P. (2024). Rapid analysis of N-nitrosamines in urine using ultra high-pressure liquid chromatography-mass spectrometry. Analytical Methods, 16(46), 8001-8009, with permission from the Royal Society of Chemistry
Shinde, S., Croft, K. D., Hodgson, J. M., & Bondonno, C. P. (2024). Rapid analysis of N-nitrosamines in urine using ultra high-pressure liquid chromatography-mass spectrometry. Analytical Methods, 16(46), 8001-8009. https://doi.org/10.1039/d4ay01870b