Document Type

Journal Article

Publication Title

Microbial genomics

Volume

8

Issue

3

PubMed ID

35316173

Publisher

Microbiology Society

School

School of Medical and Health Sciences

RAS ID

43958

Funders

Edith Cowan University National Health and Medical Research Council (Award APP1138257) Raine Medical Research Foundation (Award RPG002-19) Mahidol University

Comments

Imwattana, K., Putsathit, P., Collins, D. A., Leepattarakit, T., Kiratisin, P., Riley, T. V., & Knight, D. R. (2022). Global evolutionary dynamics and resistome analysis of Clostridioides difficile ribotype 017. 8(3), 000792. https://doi.org/10.1099/mgen.0.000792

Abstract

Clostridioides difficile PCR ribotype (RT) 017 ranks among the most successful strains of C. difficile in the world. In the past three decades, it has caused outbreaks on four continents, more than other ‘epidemic’ strains, but our understanding of the genomic epidemiology underpinning the spread of C. difficile RT 017 is limited. Here, we performed high-resolution phylogenomic and Bayesian evolutionary analyses on an updated and more representative dataset of 282 non-clonal C. difficile RT 017 isolates collected worldwide between 1981 and 2019. These analyses place an estimated time of global dissemination between 1953 and 1983 and identified the acquisition of the ermB-positive transposon Tn6194 as a key factor behind global emergence. This coincided with the introduction of clindamycin, a key inciter of C. difficile infection, into clinical practice in the 1960s. Based on the genomic data alone, the origin of C. difficile RT 017 could not be determined; however, geographical data and records of population movement suggest that C. difficile RT 017 had been moving between Asia and Europe since the Middle Ages and was later transported to North America around 1860 (95 % confidence interval: 1622–1954). A focused epidemiological study of 45 clinical C. difficile RT 017 genomes from a cluster in a tertiary hospital in Thailand revealed that the population consisted of two groups of multidrug-resistant (MDR) C. difficile RT 017 and a group of early, non-MDR C. difficile RT 017. The significant genomic diversity within each MDR group suggests that although they were all isolated from hospitalized patients, there was probably a reservoir of C. difficile RT 017 in the community that contributed to the spread of this pathogen.

DOI

10.1099/mgen.0.000792

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

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