Author Identifier
Tawfeek Khedr: https://orcid.org/0000-0003-4628-0768
Angéla Juhász: https://orcid.org/0000-0002-4317-2027
Michelle L. Colgrave: https://orcid.org/0000-0001-8463-805X
Document Type
Journal Article
Publication Title
Heliyon
Volume
11
Issue
3
Publisher
Elsevier
School
School of Science
Funders
Australian Research Council
Abstract
Inconsistent fluctuation of quinolizidine alkaloid (QA) content in lupin seeds is often observed, sometimes surpassing safe thresholds (>200 mg/kg) yet lacks a clear explanation. This study aimed to investigate the intra-plant variability of QA content in three narrow-leaf lupin genotypes: Tanjil, Quillinock, and P22660 using LC-MS/MS. QA levels in seeds from the same pods showed <30 % variation, while those from different pods in the same plant sometimes surpassed inter-plant variations (up to 94 %). The average measured QA concentrations under speed breeding conditions in Quillinock, Tanjil, and P22660 were 36, 103, and 27,000 mg/kg, respectively. The impact of longer daylight hours (16h day/8h night), a practice used in speed breeding, on QA content in the cultivar Tanjil was also studied. Longer daylight hours yielded elevated QA levels in Tanjil seeds (103 mg/kg) compared to those grown in a greenhouse (10–13h daylight, 32 mg/kg).
DOI
10.1016/j.heliyon.2025.e42367
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Comments
Khedr, T., Gao, L. L., Kamphuis, L. G., Juhász, A., & Colgrave, M. L. (2025). Intra-and inter-plant variability of quinolizidine alkaloids in narrow-leaf lupin seeds, grown under different day/night cycles. Heliyo, 11(3). https://doi.org/10.1016/j.heliyon.2025.e42367