Enhancing biobutanol production by optimizing acetone-butanol-ethanol fermentation from sorghum grains through strategic immobilization of amylolytic enzymes
Abstract
Tannin-containing sorghum grains, suitable for acetone-butanol-ethanol (ABE) production by Clostridium acetobutylicum, have required pretreatment to eliminate tannins inhibiting the strain's amylolytic activity. This study investigates biobutanol production enhancement by immobilizing enzymes on polydopamine-functionalized polyethersulfone (PES) membranes with magnetic nanoparticles for Separated Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) processes. After multi-stage hot water treatment, TG3 sorghum (from the third stage) was used, where the enzyme-immobilized PES membrane produced 4.75 g/L of ABE (3.24 g/L butanol) under SSF, 0.85 g/L under SHF, and 1.1 g/L under simple fermentation. For TG6 (from the sixth stage), 3.23, 1.29, and 1.25 g/L of ABE was produced under SSF, SHF, and simple fermentation, respectively. This enhanced performance is due to the reduced enzyme inhibition. Reusability experiments showed that the membrane retained 30 % of initial activity after three cycles. These findings suggest that enzyme-immobilized membranes can intensify ABE production and enable integrated cell recovery.
Document Type
Journal Article
Volume
419
Funding Information
Research Council of University of Isfahan
PubMed ID
39832619
School
Mineral Recovery Research Centre / School of Engineering
Copyright
subscription content
Publisher
Elsevier
Identifier
Amir Razmjou: https://orcid.org/0000-0002-3554-5129
Comments
Mehrabi, Z., Taheri-Kafrani, A., Razmjou, A., Cai, D., & Amiri, H. (2025). Enhancing biobutanol production by optimizing acetone-butanol-ethanol fermentation from sorghum grains through strategic immobilization of amylolytic enzymes. Bioresource Technology, 419. https://doi.org/10.1016/j.biortech.2025.132094